A novel experimental porcine model to assess the impact of differential pulmonary blood flow on ischemia–reperfusion injury after unilateral lung transplantation

Intensive Care Medicine Experimental

Table 3 Lung biopsies from RLL and LLL in LF and HF group

Group	LF	HF		LF	HF		LF			HF
Location	LLL		*p-value*	RLL		*p-value*	RLL	LLL	*p-value*	RLL	LLL	*p-value*
Pathological features
Interstitial widening, grade 0–3	1.5 (1–2.25)	1 (1–1.25)	0.42	1.5 (1–2.25)	1 (1–1.25)	0.42	1.5 (1–2.25)	1.5 (1–2.25)	> 0.99	1 (1–1.25)	1 (1–1.25)	> 0.99
Capillary congestion, grade 0–2	2 (2–2)	2 (1.75–2)	> 0.99	2 (1.75–2)	2 (0.75–2)	0.73	2 (2–2)	2 (1.75–2)	> 0.99	2 (0.75–2)	2 (1.75–2)	0.73
Intra-alveolar edema, grade 0–2	2 (0.75–2)	1 (0–1.25)	0.21	0 (0–1.25)	0 (0–0)	0.45	0 (0–1.25)	2 (0.75–2)	0.13	0 (0–0)	1 (0–1.25)	0.06
Hemorrhage, grade 0–2	1 (0–2)	1.5 (1–2)	0.45	2 (0.75–2)	1 (0.75–2)	0.54	2 (0.75–2)	1 (0–2)	0.47	1 (0.75–2)	1.5 (1–2)	0.68
Neutrophils in septa, grade 0–3	3 (2–3)	2 (2- 3)	0.57	1.5 (1–2)	1.5 (1–2)	> 0.99	1.5 (1–2)	3 (2–3)	0.02	1.5 (1–2)	2 (2–3)	0.08
Neutrophils intra-alveolar, grade 0–2	2 (1.75–2)	1.5 (0–2)	0.30	0.5 (0–1)	0 (0–1.25)	> 0.99	0.5 (0–1)	2 (1.75–2)	0.01	0 (0–1.25)	1.5 (0–2)	0.37

Biopsies were scored for presence of interstitial widening, capillary congestion, intra-alveolar edema, hemorrhage, neutrophils in septa, and neutrophils intra-alveolar by a pathologist blinded for experimental groups. Gradings were from 0 (considered as absent) to grade of severity (1–3); LF, low flow; HF, high flow; LLL, left lower lobe; RLL, right lower lobe; data are expressed as median (25%–75% interquartile range); and Mann–Whitney test was used for comparing the two groups