Fig. 8From: Rapid changes in mucociliary transport in the tracheal epithelium caused by unconditioned room air or nebulized hypertonic saline and mannitol are not determined by frequency of beating ciliaAn example of the effect of nebulized hypertonic saline (7% NaCl) (a) and nebulized mannitol (20% Mannitol) (b) on the mean cilia beat frequency (CBF) (black) and mucus transport velocity (MTV) (grey) across the field-of-view from a video-microscopy recording shown in Additional file 2: Video S2, Additional file 3: Video S3. The shaded area represents nebulized 7% NaCl in a and nebulized 20% mannitol in b. Different regions were used for CBF analysis in a shown as a solid black line for measurements made over the entire field-of-view and a dashed line showing measurements made in a sub-region in the top left corner or the recording, since an abundance of debris prevented robust measurement in other portions of the frameBack to article page