Fig. 1.

Model of clonidine-induced hypotension in mice. A Male C57bl/J6 mice, 10–12 weeks of age, were treated with different clonidine concentrations to identify the concentration sufficient to drop the mean arterial pressure (MAP) by half after 2 h (*p < 0.05, ns = not significant n = 3 per group). B Schematic representation of the clonidine-induced hypotension protocol used for this study to maintain MAP below 60 mmHg over 4 h. C Mice were treated with 250 ug/kg and 125 ug/kg of clonidine at indicated timepoints to maintain a MAP below 60 mmHg for at least 4 h. At 8 h, MAP values were back to normal. (*p < 0.05, ns = not significant, n = 4 per group). The dotted lines represent the mean of saline-treated mice across the timepoints