Fig. 3.

Hypotension-induced loss of GATA3 and Tie2 promotes pulmonary vascular leakage but not inflammation in vivo: A Periodic acid-Schiff (PAS) staining of paraffin-embedded lung tissue in clonidine-induced hypotensive mice and saline-treated controls. All images show bronchus (b), their corresponding arteriola (a) and the peribronchial cuffing (a surrogate of lung edema). The schematic illustration below highlights this peribronchial cuff area (in red). B Semi-quantification of peribronchial cuffing was performed by systematically surveying whole lung sections. (*p < 0.05, n = 5 per group). C Lung wet-to-dry weight ratio of saline-treated controls and clonidine-induced hypotension group (*p < 0.05, n = 8 per group). Lung mRNA for markers of vascular inflammation and tissue cytokine production (D) intercellular adhesion molecule (ICAM)-1, (E) vascular cell adhesion molecule (VCAM)-1, (F) tumor necrosis factor (TNF)ɑ, and (G) interleukin (IL)-6 (ns = not significant, n = 5–7 per group)