Fig. 1

Complement factor affects immune response and platelets function. A, B Whole blood leukocytes dropped in septic mice (both groups) after CLP. No platelet drop was found in septic fD^−/− mice (fD^−/−) 24 h after CLP, whereas platelet counts were reduced in septic wild-type (wt) at same time point. C Plasma cytokine levels of IL-6 were significant higher in septic fD^−/− mice compared to wild-type three hours after CLP. D EXTEM test were performed and clotting time (ct) in fD^−/− mice (fD-/-) was prolonged in both sham control (crtl) and after CLP. E TAT complexes were found to be higher in control (crtl) and septic fD^−/− mice. F Platelet surface expression of GPIIb/IIIa was reduced in septic fD^−/− mice compared to wild-type 24 h after CLP. Statistics: *significant to fD^−/− sham control (crtl) or wild-type (wt) sham control (crtl) with p < 0.05, (A, B, E and F) Kruskal–Wallis test (Dunn’s test correction) or (C and D) Wilcoxon–Mann–Whitney test. #Statistical significance (p < 0.05) between fD^−/− and wild-type (wt) by means of (A to F) Wilcoxon–Mann–Whitney test. Three to 12 mice/group