Fig. 1
From: Effect of noradrenaline on propofol-induced mitochondrial dysfunction in human skeletal muscle cells
Extracellular flux analysis and substrate oxidation assays. A Real-time measurement of OCR at baseline and after sequential injection of oligomycin, FCCP and antimycin A. Each data-point represents the mean of 7 subjects measured in tri- or tetraplicates. Values are normalized to mitochondrial content (CS activity). B Global mitochondrial parameters. Basal respiration, maximal respiratory capacity, ATP production and non-mitochondrial respiration determined from OCRs shown in part A. C Exogenous oxidation of fatty acids after palmitate addition to the medium during measurement. D Endogenous oxidation of fatty acids in palmitate-free medium. E CO2 production from [14C]palmitic acid (complete oxidation). F Oxidation of [14C]palmitic acid to ASM (incomplete oxidation). Error bars in each graph indicate standard error of the mean. **p < 0.01, *** p < 0.001 vs. control group. AA antimycin A, FCCP carbonyl cyanide-4- (trifluoromethoxy)phenylhydrazone, FAO fatty acid oxidation, NA noradrenaline, OCR oxygen consumption rate