Fig. 4

Co-localization of mitochondria with lysosomes. A Representative confocal images of each channel after dual staining with Mitotracker Green™ FM (accumulating in mitochondria) and LysoTracker™ Deep Red (binding to acidic lysosomes). Additionally, all cells were stained with blue-fluorescent probe NucBlue to label nuclei. Experiments were performed at least at 40 cells per each condition from 3 independent measurements (= 3 individual subjects). B Pearson’s coefficient, characterizing a degree of overlap between labelled mitochondria and lysosomes. The quantification of the co-localization was performed using ImageJ™ plugin “JACoP”. C Percent co-localization was calculated by total area of co-localized lysosomes (red channel) over total area of mitochondria (green channel). Error bars indicate standard error of the mean. *p < 0.05, **p < 0.01, ***p < 0.001 vs. control group. NA noradrenaline