Intensive Care Medicine Experimental

Table 1 Forward and reverse oligonucleotide sequences of target gene primers

From: Mild hypothermia combined with dexmedetomidine reduced brain, lung, and kidney damage in experimental acute focal ischemic stroke

Gene	Primer	Primer sequences (5′–3′)
Brain
TNF-α	Forward	CAGCCGATTTGCCATTTCATAC
TNF-α	Reverse	GGCTCTGAGGAGTAGACGATAA
IL-1β	Forward	CTATGTCTTGCCCGTGGAG
IL-1β	Reverse	CATCATCCCACGAGTCACA
ICAM-1	Forward	CTTCCGACTAGGGTCCTGAA
ICAM-1	Reverse	CTTCAGAGGCAGGAAACAGG
ZO-1	Forward	CACCACAGACATCCAACCAG
ZO-1	Reverse	CACCAACCACTCTCCCTTGT
Lung
IL-6	Forward	CTCCGCAAGAGACTTCCAG
IL-6	Reverse	CTCCTCTCCGGACTTGTGA
TNF-α	Forward	CAGCCGATTTGCCATTTCATAC
TNF-α	Reverse	GGCTCTGAGGAGTAGACGATAA
E-selectin	Forward	TGCCAAGAACAGGAATACCC
E-selectin	Reverse	CTCCCAGGATTTGAGGAACA
ICAM-1	Forward	CTTCCGACTAGGGTCCTGAA
ICAM-1	Reverse	CTTCAGAGGCAGGAAACAGG
Kidney
KIM-1	Forward	GAAGAAAACAATGGATCAAGGGAT
KIM-1	Reverse	GGAGTGGAAATGGCTCTAATGAAC
ZO-1	Forward	CACCACAGACATCCAACCAG
ZO-1	Reverse	CACCAACCACTCTCCCTTGT
Syndecan	Forward	GTTCCGCTGGTTTTGTTGTTT
Syndecan	Reverse	GATGAAGGCTGTTCCCAGGTA
IL-6	Forward	CTCCGCAAGAGACTTCCAG
IL-6	Reverse	CTCCTCTCCGGACTTGTGA
36B4	Forward	GGATCACTCAGGAGCAGGAG
36B4	Reverse	CTTGGCACTCAAGAGGAAGG

TNF tumor necrosis factor, ICAM intercellular adhesion molecule, ZO-1 zonula occludens-1, KIM kidney injury molecule-1, IL-6 interleukin-6, IL-1β interleukin-1 beta, 36B4 acidic ribosomal phosphoprotein P0

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