Effect of low-to-moderate hyperoxia on lung injury in preclinical animal models: a systematic review and meta-analysis

Minkove, Samuel; Dhamapurkar, Rhea; Cui, Xizhong; Li, Yan; Sun, Junfeng; Cooper, Diane; Eichacker, Peter Q.; Torabi-Parizi, Parizad

doi:10.1186/s40635-023-00501-x

Intensive Care Medicine Experimental

Table 1 Summary of O₂ + nonO₂ inflammatory challenge study characteristics

From: Effect of low-to-moderate hyperoxia on lung injury in preclinical animal models: a systematic review and meta-analysis

Author (year)	Country	Animals				Oxygen regimens		Inflammatory challenge		Measures reported
Author (year)	Country	Type (Strain)	Sex	Age	Wgt	FiO₂	Duration	Type	Route and Dose, timing	Measures reported
Cheney (1980)	US	Dog (Mongrel)	NR	NR	24 ± 4 kg	0.21, 0.50	4 or 8d	Oleic acid	IC; 0.09 ml/kg; − 4 h	Survival; lung injury^a; systemic and PA hemodynamics
Rinaldo (1985)	US	Rats (SD)	NR	6–8 weeks	180–230 g	0.21, 0.60	1, 3, or 6d	LPS	IP; 2.5, 7.5 mg/kg; 0 h	Survival; lung injury; lung or systemic immune response parameters^b
Garner (1988)	US	Rats (SD)	NR	NR	240–270 g	0.21, 0.40, 0.80	7d	CLP, sham	0 h	Survival; lung injury^a
Cantor (1990)	US	Hamster (Syrian Gold)	NR	NR	100 g	0.21, 0.60	4, 7, or 35d	IT elastase, NS	IT; 30U Els in 0.3 ml NS; 0.3 ml NS; 2d, − 48 h	Lung injury; lung or systemic immune response parameters, lung elastin C14 uptake
Knight (2000)	US	Rabbits (NZW)	NR	Adult	2 kg	0.21, 0.50	1d	HCL, no HCL	IT; 2.4 ml/kg; 0 h	Survival; lung injury; surfactant activity
Nara (2004)	Japan	Mice (C57BL6)	F	6–8 weeks	NR	0.21, 0.50, 0.70, 0.90	2 or 4d	L. pneumo., no infection^e	IT; 5 × 10⁶ CFU/mouse; 0 h	Survival; lung injury; lung or systemic immune response parameters; lung bacteria
Sun (2006)	China	Rats (SD)	M	NR	180-220 g	0.21, 0.40, 1.0	1d	K. pneumo, NS^e	IT; 1.3 × 10⁸ CFU/rat; NS; 0 h	Lung injury; lung or systemic immune response parameters; surfactant activity^c; blood/lung bacteria
Aggarwal (2010)	US	Mice (C57BL6)	M	6–8 weeks	NR	0.21, 0.27, 0.60	0.5, 2,3 or 4d	LPS, H₂O	IT; 0.375ug/g; sterile H₂O; − 12 h	Lung injury; lung or systemic immune responses parameters
Rodriguez-Gonzalez (2014)	Spain, Canada	Rats (SD)	M	13 weeks	257 ± 21 g	0.21, 0.40, 0.60, 1.0	1d	CLP, sham^e	0 h	Survival; lung or systemic immune response parameters; blood, lung, urine, meningeal bacteria;
Garcia-Laorden (2020)	Spain, Can	Rats (SD)	M	12–13 weeks	285 ± 21 g	0.21, 0.40, 0.60, 1.0	1d	CLP, sham, healthy	− 18 h	Survival; lung injury; lung or systemic immune response parameters; serum organ injury markers^d

BAL bronchoalveolar lavage, CLP cecal ligation and puncture, Els elastase, F female, HCL hydrochloric acid, IC intracardiac, IT intratracheal, K. pneumo. Klebsiella pneumophilia, L. pneumo Legionella pneumophilia, LPS lipopolysaccharide, M male, NR not reported, NS normal saline, NZW New Zealand White, PA pulmonary artery, ROS reactive oxygen species, SD Sprague–Dawley, Sham sham CLP, U units, UC unclear, Wgt weight, Wks weeks; 0 h, − 4 h, − 12 h, − 18 h—challenge administered at the time of or 4, 12 or 18 h before O2 therapy provided, respectively
^aLung injury measures included one or more of the following: arterial oxygen pressure; lung weights, lung wet to dry weight ratios; bronchoalveolar lavage (BAL) volume, cell, protein or albumin concentrations; alveolar permeability to solute; lung pressure/volume relationships, lung volumes, diffusion capacity; lung histologic changes; and/or Type-2 cell dysfunction
^bLung or systemic immune response parameters included one or more of the following: BAL cellularity and polymorphonuclear neutrophil cell numbers; BAL or lung tissue macrophages; BAL, lung tissue or serum cytokines, inducible nitric oxide synthase, nuclear transcription factors, apoptosis markers, S100b, reactive oxygen species, and/or myeloperoxidase measures
^cSurfactant activity measures included: BAL Type 2 cell phosphatidyl choline uptake, total phospholipids (TPL), desaturated phosphatidylcholine (DSPC), DSPC/TPL, and/or surface tension
^dSerum organ injury markers included: blood–urea–nitrogen, creatinine, aspartate or alanine aminotransferases, alkaline phosphatase, lactate dehydrogenase, troponin, serum neuron specific enolase, and/or creatine phosphokinase
^eControl challenge only administered with FiO₂ = 0.21

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