Fig. 3

Immunohistochemical analysis of Ki-67. a Representative images of the immunohistochemical staining for Ki-67 in the kidneys of sham-operated mice and mice treated with saline or rAT. Black arrows indicate Ki-67-positive cells. b Graph showing the number of Ki-67-positive cells (n = 6 in each group). c Double immunofluorescence staining for Ki-67 and CD15. White arrows indicate colocalization of Ki-67 and CD15 expression in proximal renal tubular cells. *P < 0.05 vs. Sham-operated mice. ⁺P < 0.05 vs. Saline-treated mice. Scale bars: 50 µm (b, c). HPF, high-power field