Skip to main content

Volume 2 Supplement 1


0919. Effect of catecholamine immediately after blast lung injury caused by laser-induced shock wave in a mouse model


The physical damage inflicted by blast waves is called primary blast injury, and lungs are vulnerable to blast waves [1]. Blast lung injuries (BLI) can be extremely critical during the super-acute phase, and hypotension is supposed to be the main cause of death (1) , but its etiology has not been elucidated. Recent studies have demonstrated that hypotension is mediated by the absence of vasoconstriction [2]. However, research investigated the effectiveness of catecholamine for BLI during the super-acute phase was not identified.


The present study aimed to establish a small-animal model of severe BLI using laser-induced shock wave (LISW) and to evaluate the effect of catecholamine on the super-acute phase of severe BLI.


The investigation comprised two parts. Study 1 assessed the validity of the BLI model using LISW as follows. Mice were randomly allocated to groups that received 1.2, 1.3 or 1.4 J/cm2 LISW. Survival rates, systolic blood pressure (sBP), heart rate (HR), and peripheral oxyhemoglobin saturation (SpO2) were monitored for up to 60 min thereafter and lung tissues were histopathologically analyzed. Study 2 evaluated the effects of catecholamines as follows. The mice were randomly assigned to groups that received 1.4 J/cm2 LISW followed by the immediate intraperitoneal administration of dobutamine, noradrenaline or normal saline. A sham group received no LISW or drugs. Survival rates were measured for 48 h. We also measured sBP, HR, and SpO2 before and 5 and 10 min after LISW, and left ventricular ejection fraction (EF) and systemic vascular resistance (SVR) before and 1 min after LISW.


(Study 1) The triad of BLI (hypotension, bradycardia, and hypoxemia) was evident immediately after LISW. The degree of the triad and the survival rates were aggravated with increasing doses of LISW. The histopathological findings were compatible with BLI.

(Study 2) The survival rate was highest in the group that received noradrenaline, with significantly elevated SVR and decreased EF after LISW.


The LISW induced lung injury model seems to be useful as severe BLI in small animals without any large scaled equipment. The main cause of death during the super-acute phase of severe BLI might be hypotension due to the absence of peripheral vasoconstriction. The immediate administration of an α1-adrenergic receptor agonist such as noradrenaline right after exposure to blast waves might be an effective treatment during the super-acute phase of severe BLI.


  1. Guy RJ, et al.: Physiologic responses to primary blast. The Journal of Trauma 1998,45(6):983–987. 10.1097/00005373-199812000-00001

    Article  CAS  PubMed  Google Scholar 

  2. Ohnishi M, et al.: Reflex nature of the cardiorespiratory response to primary thoracic blast injury in the anaesthetised rat. Experimental Physiology 2001,86(3):357–364. 10.1113/eph8602145

    Article  CAS  PubMed  Google Scholar 

Download references

Grant acknowledgment

This work was supported by a grant-in-aid for the Special Research Program from the National Defense Medical College (D.S.).

Author information

Authors and Affiliations


Rights and permissions

Open Access  This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.

The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.

To view a copy of this licence, visit

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Miyawaki, H., Saitoh, D., Hagisawa, K. et al. 0919. Effect of catecholamine immediately after blast lung injury caused by laser-induced shock wave in a mouse model. ICMx 2 (Suppl 1), O27 (2014).

Download citation

  • Published:

  • DOI: