- Oral presentation
- Open Access
0030. Effect of exercise training on muscle function in a recovery model of critical illness
© Sigurta et al; licensee Springer. 2014
- Published: 26 September 2014
- Exercise Training
- Grip Strength
- Exercise Capacity
- Critical Illness
- Muscle Function
Survivors of critical illness experience significant skeletal muscle weakness and physical disability, which may persist for years . Early mobilization is being encouraged to improve functional outcomes . Decreased mitochondrial function and altered mitochondrial biogenesis are implicated in the pathogenesis of sepsis-induced muscle dysfunction . Mitochondrial biogenesis can be stimulated by physical activity .
To assess the role of exercise training on muscle function and mitochondrial biogenesis in a long term rat model of critical illness and recovery.
Peritonitis was induced in male Wistar rats by i.p. injection of the fungal cell wall product, zymosan. Animals were divided into 2 groups: (i) trained animals who underwent daily motorised treadmill sessions from day 2-14, progressively increasing treadmill speed and duration to 30 mins at 30 cm/s; (ii) control animals.
Weight and clinical score were recorded daily. Muscle function was assessed on days 2, 7 and 14 using exercise capacity and forelimb grip strength. On day 14, animals were culled for harvesting of gastrocnemius and soleus muscle that were weighed and then used to measure (by RT-PCR) gene expression assays of the biogenesis factors, PGC-1alpha, NRF and Tfam. Results given as ratios, using HMBS as the housekeeping gene.
Exercise training increases weight gain in this model of critical illness and recovery. Preliminary data shows improved mitochondrial biogenesis in gastrocnemius but not soleus with exercise.
- Herridge MS, et al.: Functional disability 5 years after acute respiratory distress syndrome. N Engl J Med 2011, 364: 1293–304. 10.1056/NEJMoa1011802PubMedView ArticleGoogle Scholar
- Schweickert WD, et al.: Early physical and occupational therapy in mechanically ventilated, critically ill patients: a randomised controlled trial. Lancet 2009, 373: 1874–82. 10.1016/S0140-6736(09)60658-9PubMedView ArticleGoogle Scholar
- Carré JE, et al.: Survival in critical illness is associated with early activation of mitochondrial biogenesis. Am J Respir Crit Care Med 2010, 182: 745–51. 10.1164/rccm.201003-0326OCPubMed CentralPubMedView ArticleGoogle Scholar
- Wang L, et al.: Similar expression of oxidative genes after interval and continuous exercise. Med Sci Sports Exerc 2009, 41: 2136–44. 10.1249/MSS.0b013e3181abc1ecPubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.