Volume 2 Supplement 1
0030. Effect of exercise training on muscle function in a recovery model of critical illness
© Sigurta et al; licensee Springer. 2014
Published: 26 September 2014
Survivors of critical illness experience significant skeletal muscle weakness and physical disability, which may persist for years . Early mobilization is being encouraged to improve functional outcomes . Decreased mitochondrial function and altered mitochondrial biogenesis are implicated in the pathogenesis of sepsis-induced muscle dysfunction . Mitochondrial biogenesis can be stimulated by physical activity .
To assess the role of exercise training on muscle function and mitochondrial biogenesis in a long term rat model of critical illness and recovery.
Peritonitis was induced in male Wistar rats by i.p. injection of the fungal cell wall product, zymosan. Animals were divided into 2 groups: (i) trained animals who underwent daily motorised treadmill sessions from day 2-14, progressively increasing treadmill speed and duration to 30 mins at 30 cm/s; (ii) control animals.
Weight and clinical score were recorded daily. Muscle function was assessed on days 2, 7 and 14 using exercise capacity and forelimb grip strength. On day 14, animals were culled for harvesting of gastrocnemius and soleus muscle that were weighed and then used to measure (by RT-PCR) gene expression assays of the biogenesis factors, PGC-1alpha, NRF and Tfam. Results given as ratios, using HMBS as the housekeeping gene.
Exercise training increases weight gain in this model of critical illness and recovery. Preliminary data shows improved mitochondrial biogenesis in gastrocnemius but not soleus with exercise.
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