Volume 2 Supplement 1

ESICM LIVES 2014

Open Access

0078. Establishing a detailed short-term rat model of partial ischaemia/reperfusion injury

  • G Sabbatini1,
  • A Dyson1 and
  • M Singer1
Intensive Care Medicine Experimental20142(Suppl 1):P1

https://doi.org/10.1186/2197-425X-2-S1-P1

Published: 26 September 2014

Introduction

Liver ischaemia/reperfusion (I/R) injury may be observed after major hepatic surgery or resuscitation from severe trauma/haemorrhage. Well-characterised and representative animal models are needed to better understand mechanisms of injury, apply effective treatments and prevent complications.

Objectives

To establish a well-characterized rat model of partial liver I/R injury.

Methods

Under isoflurane anaesthesia, tracheotomized male Wistar rats underwent left common carotid artery and right jugular vein instrumentation for BP measurement/blood sampling and fluid infusion (10 ml/kg/hr), respectively, and bladder catheterization for urine output measurement. Via a transverse subcostal laparotomy, blood vessels to the median and left liver lobes were occluded with a surgical clamp for 60 mins. On release of the clamp, the remaining liver lobes were ligated to prevent a steal phenomenon. The animals were observed for a further 5 hours. Sham animals underwent the same procedure except for vascular occlusion. Measurements were made of haemodynamics (BP, echocardiography), blood gas analysis, and biochemical and functional (Indocyanine Green plasma disappearance rate, ICG-PDR) liver function tests. A tissue PO2 probe (tPO2) (Oxford Optronix, UK) was placed in contact with liver tissue to measure hepatic tissue PO2. Tissue samples were taken to assess microscopic and ultrastructural injury both locally and remotely (data not shown).

Results

See figure 1.
Figure 1

ISC= Ischaemia, BL= baseline, OD= optical density AST= aspartate aminotransferase. Data shown as mean (SEM) (A-E) or median (interquartile range) (F). Sham= 6, Ischaemia= 5/group, *p< 0.05 comparing ischaemia to sham, #p< 0.05 comparing BL to 5h. Statistics performed using t-test or two-way ANOVA and Bonferroni's test for multiple comparisons, as appropriate.

Liver ischaemia resulted in an increase of markers of hepatic injury (AST) and function (ICG-PDR and Lactate). Cardiac output was maintained but arterial base excess was different between groups. tPO2 at 5 hours post-reperfusion recovered in shams but not in I/R group.

Conclusions

This severe liver I/R model demonstrates derangement of hepatic function, biochemistry, haemodynamic and ultrastructure. It offers utility for the assessment of interventions aimed at preventing or reducing I/R injury.

Authors’ Affiliations

(1)
University College London, Bloomsbury Institute of Intensive Care Medicine

References

  1. Eguchi S, Kamlot A, Ljubimova J, Hewitt WR, Lebow LT, Demetriou AA, Rozga J: Fulminant hepatic failure in rats: survival and effect on blood chemistry and liver regeneration. Hepatology 1996,24(6):1452–1459. 10.1002/hep.510240626PubMedView ArticleGoogle Scholar
  2. Merle U, Sieg O, Stremmel W, Encke J, Eisenbach C: Sensitivity and specificity of plasma disappearance rate of indocyanine green as a prognostic indicator in acute liver failure. BMC Gastroenterology 2009, 9: 91. 10.1186/1471-230X-9-91PubMed CentralPubMedView ArticleGoogle Scholar

Copyright

© Sabbatini et al; licensee Springer. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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