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Volume 2 Supplement 1


  • Poster presentation
  • Open Access

0295. Induction and repression effects of heat shock (HS) and LPS and modulatory effects of glutamine on blood mononuclear cells -hsprotein-72 from icu patients with severe sepsis, trauma and healthy controls

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Intensive Care Medicine Experimental20142 (Suppl 1) :P17

  • Published:


  • Public Health
  • Flow Cytometry
  • Inflammatory Response
  • Fluorescence Intensity
  • Glutamine


In severe sepsis (SS) or trauma-related systemic inflammatory response syndrome (SIRS), induction of heat-shock-protein-72 (HSP72) may protect cells from stress.


We compared the heat-shock (HS) with the lipopolysaccharide (LPS) induction/repression effect on HSP72 of peripheral blood mononuclear cells (PBMCs) in SS or SIRS patients and healthy-controls (H) and investigated any possible modulating glutamine (Gln) effect.


PBMCs from 16/H, 11/SS, and 7/SIRS were incubated with 1µg/ml LPS or 43o HS vs. no stimulation for 4h. In each group 3 experiments involved L-Ala-Gln10mM incubation 1h before (Gln-b) or after (Gln-a) induction, or no glutamine (1088 measurements). Intracellular Mean Fluorescence Intensity (MFI) levels of monocytes (mHSP72) or lymphocytes (lHSP72) were determined using Flow Cytometry.


In H-PBMCs, LPS did not affect mHSP72 (79±10 MFI vs. 78±13) or lHSP72 (7±1.7 vs. 7±2). HS induced mHSP72 (454±60, p< 0.0001) and lHSP72 (41±7, p< 0.0001) with or without Gln (p< 0.0001). Basal mHSP72 was higher in SIRS compared to H (144±25 vs. 78±10, p< 0.03). A HS-induction effect on SIRS-mHSP72 (394±108, p< 0.04) and lHSP72 (37±5, p< 0.02) was further enhanced by Gln-b (495±114, p< 0.01 and 58±14, p< 0.04). LPS suppressed SIRS-mHSP72 (120±54 vs. 144±25, p< 0.02) especially in the Gln-b group (107±19, p< 0.02). Basal Gln-b mHSP72 in SS was higher compared to H (112±16 vs. 69±10, p< 0.03). In SS-PBMCs HS, but not LPS, induced mHSP72 (492±56 vs. 108±19, p< 0.003). LPS repressed the SS-lHSP72 (10±2 vs. 17±2, p< 0.007) an effect attenuated by Gln-b (13±5).


Heat shock greatly induces mHSP72 and lHSP72 of ICU patients' PBMCs. LPS may repress lHSP72 in septic or trauma patients. Glutamine pre-treatment may either enhance HS-induction or LPS-repression on mHSP72 or attenuate LPS-repression on lHSP72 in SS and SIRS groups.


Grant acknowledgment

This research has been co-financed by the European Union (European Social Fund (ESF)) and Greek national funds through the Operational Program ''Education and Lifelong Learning'' of the National Strategic Reference Framework (NSRF)-Research Funding Program: THALES.

Authors’ Affiliations

1st Department of Propaedeutic InternalMedicine, University of Athens, Athens, Greece
Department of Immunology - Histocompatibility, Specialized Center & Referral Center for Primary Immunodeficiencies - Paediatric Immunology, “Aghia Sophia” Children's Hospital, Athens, Greece
First Critical Care Department, University of Athens, Evangelismos Hospital, Athens, Greece
PICU, University of Crete, University Hospital, Heraklion, Greece


© Briassouli et al; licensee Springer. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.