- Poster presentation
- Open Access
0589. Pravastatin exerts opposite effects on splanchnic microcirculatory oxygenation during sham or septic conditions in an animal model of polymicrobial sepsis
Intensive Care Medicine Experimental volume 2, Article number: P34 (2014)
The aim of this study was to evaluate the effects of pravastatin on the microcirculatory oxygenation of the colon in a rodent model of polymicrobial sepsis.
The data derive from a total of 40 experiments on rats studied with approval of the local animal care and use committee. Pravastatin (0.2 mg/kg) or NaCl were injected subcutaneously 18 h prior to sepsis induction (colon ascendens stent peritonitis) or sham operation. 24 h after induction of sepsis the animals were re-laparotomized under general anaesthesia and received ongoing fluid replacement and pressure-limited ventilation for 120 min. Macrohemodynamic variables were recorded and microcirculatory oxygen supply (µDO2) and post-capillary oxygen saturation (µHbO2) of the colon were measured simultaneously via laser Doppler and tissue reflectance spectrophotometry, respectively. Data are presented as means±SD, 2-way ANOVA followed by Dunnett (vs. baseline) or Tukey (between groups).
1.) In pravastatin pre-treated sham animals the microcirculatory oxygenation µHbO2 declined by 9.8±9.4% with no change in the NaCl group. Figure 1.
2.) During sepsis pravastatin pre-treatment ameliorated the deterioration of µHbO2 (-5.5±8.2%), compared to a significant decrease in the NaCl group (-8.4±8.7%). Figure 2.
3.) Macrohaemodynamic variables and microcirculatory oxygen supply of the colon did not differ between the groups.
Pravastatin has opposite effects on splanchnic microcirculatory oxygenation depending on septic or non-septic conditions. These effects are independent of the macrocirculation or microcirculatory oxygen supply.
McGown CC, Brookes ZL: Beneficial effects of statins on the microcirculation during sepsis: the role of nitric oxide. Br J Anaesth 2007, 98: 163–175. 10.1093/bja/ael358
La Mura V, Pasarin M, Meireles CZ, Miquel R, Rodriguez-Vilarrupla A, Hide D, Gracia-Sancho J, Garcia-Pagan JC, Bosch J, Abraldes JG: Effects of simvastatin administration on rodents with lipopolysaccharide-induced liver microvascular dysfunction. Hepatology 2013, 57: 1172–1181. 10.1002/hep.26127
Tehrani S, Mobarrez F, Lins PE, Adamson U, Wallen HN, Jorneskog G: Impaired endothelium-dependent skin microvascular function during high-dose atorvastatin treatment in patients with type 1 diabetes. Diabetes & vascular disease research 2013, 10: 483–488. 10.1177/1479164113491275
About this article
Cite this article
Beck, C., Barthel, F., Herminghaus, A. et al. 0589. Pravastatin exerts opposite effects on splanchnic microcirculatory oxygenation during sham or septic conditions in an animal model of polymicrobial sepsis. ICMx 2, P34 (2014). https://doi.org/10.1186/2197-425X-2-S1-P34
- Sham Animal
- Microvascular Function
- Septic Condition
- Polymicrobial Sepsis