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0987. FAS activation alters tight junction proteins in pulmonary alveolar epithelial cells
© Herrero et al; licensee Springer. 2014
- Published: 26 September 2014
- Acute Respiratory Distress Syndrome
- Tight Junction Protein
- Alveolar Epithelium
- Intratracheal Instillation
- Lung Edema
Active soluble Fas ligand (sFasL) accumulates in lung fluid of patients with acute respiratory distress syndrome (ARDS), and causes apoptosis and inflammation in lung epithelial cells . Alveolar epithelial damage induced by Fas receptor activation results in protein-rich lung edema . Dysfunction of the tight junction proteins may contribute to the formation of lung edema.
Determine whether sFasL increases protein permeability of the alveolar epithelium by mechanisms involving disruption of the tight junction proteins in ARDS.
Primary human pulmonary alveolar epithelial cells were cultured in permeable transwell chambers. After reaching maximal confluency, the cells were incubated for 0.5, 1, 2 or 4 h with medium with or without human recombinant sFasL (rh-sFasL). Protein permeability of the cell monolayer was measured by using fluorescein-labeled albumin (FITC-Albumin). C56BL/6 wild-type mice and lpr (Fas deficient) mice were treated with an intratracheal dose of rh-sFasL (25 ng/g b.w.) or PBS, and the lungs were studied 16 h later. We performed immunofluorescence double staining for the detection of tight junction proteins (ZO-1 and Occludin) and apoptosis (Terminal Transferase dUTP Nick End Labeling assay).
In vitro, human sFasL increased protein permeability of the alveolar epithelial cell monolayer (medium only: 17.17 ± 2.4% vs rh-sFasL: 28.0 ± 3.6%, means ± SD, p< 0.05, t-test), altered the distribution of the tight junction proteins ZO-1 and Occludin, and induced apoptosis. In vivo, intratracheal instillation of rh-sFasL, which increases pulmonary protein permeability in wild-type but not in lpr mice, altered the distribution of ZO-1 and Occludin, and induced apoptosis in cells of the alveolar walls only in wild-type but not in lpr mice.
Activation of the Fas/FasL system increased protein permeability of the pulmonary alveolar epithelium in vitro and in vivo. This increased permeability was associated with disruption of tight junctions and apoptosis. These results provide a mechanism that could be targeted for the prevention of lung edema in ARDS.
FIS 12/02451, FIS 12/02898, FIS 11/02791.
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