1069. Pharmacological preconditioning with vitamin c attenuates intestinal injury via the induction of heme oxygenase-1 after hemorrhagic shock in rats
© Zhao et al; licensee Springer. 2014
Published: 26 September 2014
Pre-induction of heme oxygenase (HO)-1, which is regarded as an effective method of “organ preconditioning”, exerts beneficial effects during hemorrhagic shock (HS). However, the available HO-1 inducers exhibit disadvantages such as toxicity or complex technical requirements. Therefore, a safe and convenient HO-1 inducer would be promising and could be exploited in the treatment of foreseeable hemorrhaging, such as prior to major surgery. Recently, vitamin C (VitC) has been shown to attenuate organ injuries and inhibit inflammatory responses in hemorrhagic shock , but the specific mechanism remains unclear. Studies on the relationship between HO-1 and VitC are limited, and the results are controversial[4, 5].
We investigated the effect of vitamin C (VitC) on intestinal HO-1 expression and the involved mechanism. We further investigated if VitC pretreatment prevented HS related intestinal tissue injuries via HO-1 induction.
The IEC-6 were treated with grade concentration of VitC as well as SB203580, PD98059 and SP600125, the inhibitors of p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase (ERK) 1/2 and c-Jun N-terminal kinase (JNK). SD rats were pretreated with VitC (intraperitoneally, 100mg/Kg), HS was induced by drawing blood from the rat femoral artery (mean arterial pressure = 30 mm Hg) for 1 hr and resuscitating with the shed blood and Ringer's solution. Some rats further received zinc protoporphyrin (Znpp, intraperitoneally, 3 mg/kg), a HO-1 inhibitor.
These data suggests VitC might be applied as a safe inducer of intestinal HO-1 and VitC pretreatment attenuated HS related intestinal injuries via induction of HO-1 by activating ERK1/2 pathway.
This work is supported by National Natural Science Foundation of China projects 81171789
- Kubulus D, et al.: Shock. 2008,29(5):583–590.PubMedGoogle Scholar
- Raddatz A, et al.: American journal of respiratory and critical care medicine. 2006,174(2):198–207. 10.1164/rccm.200508-1221OCPubMedView ArticleGoogle Scholar
- Van PY, et al.: The Journal of trauma. 2011,71(1):20–24. discussion 24–25 10.1097/TA.0b013e3182214f44PubMedView ArticleGoogle Scholar
- Kim JY, Lee SM: Life sciences. 2004,75(16):2015–2026. 10.1016/j.lfs.2004.06.002PubMedView ArticleGoogle Scholar
- Elbekai RH, et al.: Cancer letters. 2007,246(1–2):54–62. 10.1016/j.canlet.2006.01.029PubMedView ArticleGoogle Scholar
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