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Volume 3 Supplement 1

ESICM LIVES 2015

  • Poster presentation
  • Open Access

Heparin effect in alveolar cells and macrophages in an acute lung injury model

  • 1,
  • 2,
  • 3,
  • 1,
  • 4,
  • 4,
  • 1,
  • 1,
  • 5,
  • 1, 2 and
  • 2, 5
Intensive Care Medicine Experimental20153 (Suppl 1) :A570

https://doi.org/10.1186/2197-425X-3-S1-A570

  • Published:

Keywords

  • Heparin
  • Acute Lung Injury
  • Alveolar Macrophage
  • Acute Respiratory Distress Syndrome
  • Alveolar Cell

Introduction

Acute Lung Injury (ALI) and Acute Respiratory Distress Syndrome (ARDS) are characterized by a promptly release of proinflammatory mediators that downregulate natural anticoagulant mechanisms, initiate the coagulation system, impair fibrinolysis and produce the rupture of the endothelial and epithelial monolayer [1]. Inflammation of ALI/ARDS is initiated and strongly regulated by proinflammatory activation of macrophages, which lead to the recruitment of other inflammatory cells, producing alveolar cells injury, propagating the coagulant response and promoting the injury. Currently there is no effective treatment for this disease. Previous studies have presented the beneficial effect of anticoagulants, not only for their anticoagulation activity but also for their antiinflammatory action [2],[3].

Objectives

To evaluate the effect of the treatment with heparin in human alveolar cells and alveolar macrophages after induce an injury, mimicking an in-vitro ALI.

Methods

Human alveolar primary cells and alveolar macrophages from pulmonary biopsies were isolated and seeded. Sodium non-fractioned heparin (100 UI/ml) was administered to the cells after the induction of an injury with a pro-inflammatory stimulus (Cytomix: mix of TNFα, IL1β and IFNγ; 25 ng/ml or Lipopolysaccharide protein from Escherichia coli 055 : B5; 1000 ng/ml). The effect of heparin was assessed by the analysis of proinflammatory markers (IL12p40, iNOS, TNFα and IL-1β) and anti-inflammatory markers (IL10 and IL13), cell proliferation and permeability (measuring transmembrane resistance). Data are expressed as mean ± SEM (units are relative to the expression of control group). Statistical analysis was performed using One-Way-ANOVA and post-hoc (Newman Keuls) test. Statistical significance p ≤ 0.05 is considered.

Results

Heparin was able to modify the inflammatory response of both cell populations, decreasing it significantly (p ≤ 0.05) in the case of macrophages (iNOS: Control:1 ± 0.09, Injured group:41.68 ± 4.86, Heparin group:0.54 ± 0.06. IL12p40: Control:1 ± 0.11, Injured group:46.74 ± 4.32, Heparin group:0.15 ± 0.009). The permeability of the monolayer and cell proliferation of alveolar cells did not show changes.

Conclusions

Heparin has an immunomodulatory effect in alveolar cells and reduces inflammation in macrophages. This mechanism produced by heparin could have a beneficial effect in ALI.

Declarations

Grant Acknowledgment

PI12/02548, Fundació Parc Taulí and CIBERES

Authors’ Affiliations

(1)
Fundación Parc Taulí, Sabadell, Spain
(2)
Ciber de Enfermedades Respiratorias, Sabadell, Spain
(3)
Ponchaillou University Hospital, Surgical Intensive Care, Rennes, France
(4)
University Hospital of Mutua de Terrassa, University of Barcelona, Thoracic Surgery Service, Terrassa, Spain
(5)
Corporación Sanitaria y Universitaria Parc Taulí, Critical Care Center, Sabadell, Spain

References

  1. Ware LB, Matthay MA: The acute respiratory distress syndrome. N Engl J Med. 2000, 342: 1334-1349. 10.1056/NEJM200005043421806.PubMedView ArticleGoogle Scholar
  2. Mu E, Ding R, An X, Li X, Chen X, Ma X: Heparin attenuates lipopolysaccharide-induced acute lung injry by inhitibiting nitric oxide synthase and TGF-β/Smad signaling pathway. Thromb Res. 2012, 129 (4): 479-485. 10.1016/j.thromres.2011.10.003.PubMedView ArticleGoogle Scholar
  3. Li Y, Sun JF, Cui X, Mani H, Danner RL, Li X, et al: The effect of heparin administration in animal models of sepsis: A prospective study in Escherichia coli-challenged mice and a systematic review and metaregression analysis of published studies. Crit Care Med. 2011, 39 (5): 1104-1112. 10.1097/CCM.0b013e31820eb718.PubMedPubMed CentralView ArticleGoogle Scholar

Copyright

© Camprubí-Rimblas et al.; 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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