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Volume 3 Supplement 1


Effects of low dose intravenous sodium nitrite on arterial oxygenation and hemodynamics in experimental acute respiratory distress syndrome (ARDS)


Nitrite (NO2-) is an endogenous storage pool for nitric oxide (NO) [1]. We showed that sodium nitrite (NaNO2) mitigates ventilator-induced lung injury via NO dependent mechanisms in rats [2].


We hypothesized that low dose intravenous (i.v.) NaNO2 may improve arterial oxygenation and reduce mean pulmonary artery pressure (MPAP) and pulmonary vascular resistance (PVR) in ARDS in pigs.


ARDS was induced in 12 pigs by surfactant depletion due to saline lung lavages [3]. Two groups were investigated for 5 h: 1. Controls (n = 6) and 2. NaNO2 i.v. (0.3 mg/kg BW bolus, followed by 0.1725 mg/kg BW continuously; n = 6). We measured mean arterial pressure (MAP), MPAP and cardiac output as well as exhaled NO (NOex), blood gases and Wet/Dry-Ratios of lung tissue.


At baseline the arterial oxygen tension (PaO2) was 539 ± 50 mmHg and 508 ± 35 mmHg in Controls and NaNO2 i.v. respectively (fraction of inspired oxygen = 1.0). PaO2 decreased to 67 ± 17 mmHg (Controls) and 57 ± 13 mmHg (NaNO2 i.v.) after ARDS induction. During the protocol, PaO2 increased to 120 ± 73 mmHg (Controls) and 103 ± 82 mmHg (NaNO2 i.v.). NOex was unchanged in both groups. Lung Wet/Dry-Ratios were 8.1 ± 0.8 (Controls) and 8.9 ± 0.7 (NaNO2 i.v.). For hemodynamic values see Table 1 (all values: mean ± SD).

Table 1


Lung lavage induced severe ARDS with increased MPAP and PVR in both groups. I.v. application of low dose NaNO2 did not reduce lung edema formation and did not improve arterial oxygenation or pulmonary hemodynamics in this model of severe ARDS in pigs.

Grant Acknowledgment

This study was supported by the Deutsche Forschungsgemeinschaft (PI795/2-1).


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Kronfeldt, S., Lother, P., Francis, R. et al. Effects of low dose intravenous sodium nitrite on arterial oxygenation and hemodynamics in experimental acute respiratory distress syndrome (ARDS). ICMx 3 (Suppl 1), A574 (2015).

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