Skip to main content

Advertisement

Volume 3 Supplement 1

ESICM LIVES 2015

Intravenous oxygen administration in a rat model of hypoxia

Article metrics

Introduction

Hypoxemia reduces tissue oxygen delivery, thus compromising cell metabolism and organ function. Supplemental oxygen at high concentrations may prove ineffective and issues relating to hyperoxia, barotrauma, mechanical ventilation and extracorporeal oxygenation are well documented [1, 2]. A century ago, Tunnicliffe et al reported rapid and safe relief of cyanosis in patients by administration of intravenous oxygen gas [3]. This re-discovered route warrants re-exploration.

Objectives

To test the safety and efficacy of intravenous administration of oxygen either as a pure gas or dissolved in Ringer's Lactate (RL) solution saturated to 100%.

Methods

Under isoflurane anesthesia, male Wistar rats (about 300 g bw) underwent arterial and central venous cannulation, tracheotomy, bladder cannulation and placement of tissue PO2 probes (Oxford Optronix, Oxford, UK) in leg muscle and liver. Hypoxia was induced by breathing a hypoxic gas mix (FiO2 0.1). At 60 minutes, a continuous iv infusion of pure O2 gas (2 mL/kg/h) or oxygenated RL (10 mL/kg/h) was begun. An equal volume of normal RL was given to controls. Echocardiography, arterial blood gas analysis, mean arterial pressure (MAP), urine output, muscle and liver tPO2 were measured at baseline and at hourly intervals for 4 hours.

Results

Infusion of pure O2 gas caused early death due to pulmonary embolism so this technique was abandoned. Administration of oxygenated RL (PO2 of solution at end-experiment = 87.5 ± 1.7 kPa) was however safe but did not produce any significant increase in PaO2 or SaO2, in comparison to controls. However, O2 delivery, MAP (Figure 1) and liver PO2 (Figure 2) (but not muscle PO2) rose progressively with oxygenated RL with urine output increasing to supranormal values (Figure 3).

Figure 1
figure1

Mean arterial pressure.

Figure 2
figure2

tPO 2 (liver).

Figure 3
figure3

Urine output.

Conclusions

In this rat model of hypoxia, the intravenous infusion of oxygenated RL was safe. While it did not produce any increase in arterial or muscle oxygenation, it did appear to impact on the splanchnic circulation, increasing liver PO2 and urine output.

References

  1. 1.

    Budinger GRS, Mutlu GMl: Balancing the Risks and Benefits of Oxygen Therapy in Critically III Adults. Chest. 2013, 143 (41): 1151-1162.

  2. 2.

    Tramm R, Ilic D, Davies AR, Pellegrino VA, Romero L, Hodgson C: Extracorporeal membrane oxygenation for critically ill adults. Cochrane Database Syst Rev. 2015, 1: CD010381-

  3. 3.

    Tunnicliffe FW, Stebbing GF: The intravenous injection of oxygen gas as a therapeutic measure. Lancet. 1916, 188 (4851): 321-323. 10.1016/S0140-6736(00)97237-4.

Download references

Author information

Correspondence to E Damiani.

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0), which permits use, duplication, adaptation, distribution, and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Keywords

  • Mean Arterial Pressure
  • Urine Output
  • Muscle Oxygenation
  • Venous Cannulation
  • Splanchnic Circulation