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Volume 3 Supplement 1


  • Poster presentation
  • Open Access

Intravenous oxygen administration in a rat model of hypoxia

  • 1, 2,
  • 1 and
  • 2
Intensive Care Medicine Experimental20153 (Suppl 1) :A575

  • Published:


  • Mean Arterial Pressure
  • Urine Output
  • Muscle Oxygenation
  • Venous Cannulation
  • Splanchnic Circulation


Hypoxemia reduces tissue oxygen delivery, thus compromising cell metabolism and organ function. Supplemental oxygen at high concentrations may prove ineffective and issues relating to hyperoxia, barotrauma, mechanical ventilation and extracorporeal oxygenation are well documented [1, 2]. A century ago, Tunnicliffe et al reported rapid and safe relief of cyanosis in patients by administration of intravenous oxygen gas [3]. This re-discovered route warrants re-exploration.


To test the safety and efficacy of intravenous administration of oxygen either as a pure gas or dissolved in Ringer's Lactate (RL) solution saturated to 100%.


Under isoflurane anesthesia, male Wistar rats (about 300 g bw) underwent arterial and central venous cannulation, tracheotomy, bladder cannulation and placement of tissue PO2 probes (Oxford Optronix, Oxford, UK) in leg muscle and liver. Hypoxia was induced by breathing a hypoxic gas mix (FiO2 0.1). At 60 minutes, a continuous iv infusion of pure O2 gas (2 mL/kg/h) or oxygenated RL (10 mL/kg/h) was begun. An equal volume of normal RL was given to controls. Echocardiography, arterial blood gas analysis, mean arterial pressure (MAP), urine output, muscle and liver tPO2 were measured at baseline and at hourly intervals for 4 hours.


Infusion of pure O2 gas caused early death due to pulmonary embolism so this technique was abandoned. Administration of oxygenated RL (PO2 of solution at end-experiment = 87.5 ± 1.7 kPa) was however safe but did not produce any significant increase in PaO2 or SaO2, in comparison to controls. However, O2 delivery, MAP (Figure 1) and liver PO2 (Figure 2) (but not muscle PO2) rose progressively with oxygenated RL with urine output increasing to supranormal values (Figure 3).
Figure 1
Figure 1

Mean arterial pressure.

Figure 2
Figure 2

tPO 2 (liver).

Figure 3
Figure 3

Urine output.


In this rat model of hypoxia, the intravenous infusion of oxygenated RL was safe. While it did not produce any increase in arterial or muscle oxygenation, it did appear to impact on the splanchnic circulation, increasing liver PO2 and urine output.

Authors’ Affiliations

Biomedical Sciences and Public Health, Università Politecnica delle Marche, Ancona, Italy
University College London, Bloomsbury Institute of Intensive Care Medicine, London, United Kingdom


  1. Budinger GRS, Mutlu GMl: Balancing the Risks and Benefits of Oxygen Therapy in Critically III Adults. Chest. 2013, 143 (41): 1151-1162.PubMedPubMed CentralView ArticleGoogle Scholar
  2. Tramm R, Ilic D, Davies AR, Pellegrino VA, Romero L, Hodgson C: Extracorporeal membrane oxygenation for critically ill adults. Cochrane Database Syst Rev. 2015, 1: CD010381-PubMedGoogle Scholar
  3. Tunnicliffe FW, Stebbing GF: The intravenous injection of oxygen gas as a therapeutic measure. Lancet. 1916, 188 (4851): 321-323. 10.1016/S0140-6736(00)97237-4.View ArticleGoogle Scholar


© Damiani et al.; 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.