Skip to main content

Advertisement

Volume 3 Supplement 1

ESICM LIVES 2015

Tissue and plasma putrescine levels in non-survivors of sepsis in a fluid-resuscitated rat model of faecal peritonitis

Article metrics

  • 360 Accesses

Introduction

The polyamine, putrescine, was first isolated from putrefying meat but is thought to play an important role in cell growth and differentiation ([1]). It also generates succinate via GABA and can thus serve as an energy source to the small intestine ([2]). Elevated plasma putrescine levels have been reported in an endotoxic rodent model ([3]). We have previously characterized a 72 h fluid-resuscitated rat model of faecal peritonitis where prognostication can be accurately made as early as 6 h post-insult ([4]).

Objectives

Using this long-term sepsis model, to assess differences in liver and plasma levels of putrescine in predicted survivors and non-survivors.

Methods

Awake, instrumented yet fully mobile male Wistar rats (325 ± 15 g) received an i.p. injection of 4µl/g faecal slurry. Fluid resuscitation (50:50 mix of 5% glucose/Hartmann's; 10 ml/kg/h) was commenced at 2 h. At 6 h, an echo-measured heart rate cut-off of 460 bpm was used to classify animals into predicted survivors or non-survivors. Animals were sacrificed at 6 h, 24 h or 72 h for liver and blood sampling. A group of control animals were treated identically but without injection of faecal slurry. Putrescine levels were measured using mass spectrometry. Results were analysed using two-way ANOVA and post-hoc testing and considered statistically significant when p < 0.05.

Results

In this model septic animals had a mortality rate of 56% with death occurring between 18-36 h. At 6 h septic animals displayed only mild clinical features of illness. However, even as early as 6 h, significant differences were noted in putrescine levels in liver and plasma from non-surviving septic animals.

Conclusions

An association was seen between eventual non-survival and elevated putrescine levels in both liver and plasma at both 6 h and 24 h. The significance of this finding warrants further investigation.

Table 1 Table 1

Grant Acknowledgement

ESICM Basic Science Award, Intensive Care Foundation (UK), NIHR

References

  1. 1.

    Tabor CW, et al: Ann Rev Biochem. 1984, 53: 747-90.

  2. 2.

    Bardocz S, et al: Gut. 1998, 42: 24-48. 10.1136/gut.42.1.24.

  3. 3.

    Lortie MJ, et al: Am J Physiol - Cell Physiol. 2000, 278: C1191-9.

  4. 4.

    Rudiger A, et al: Clin Sci. 2013, 124: 391-401. 10.1042/CS20120334.

Download references

Author information

Correspondence to DT Andreis.

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0), which permits use, duplication, adaptation, distribution, and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Andreis, D., Khaliq, W., Neugebauer, S. et al. Tissue and plasma putrescine levels in non-survivors of sepsis in a fluid-resuscitated rat model of faecal peritonitis. ICMx 3, A616 (2015) doi:10.1186/2197-425X-3-S1-A616

Download citation

Keywords

  • Small Intestine
  • Peritonitis
  • Rodent Model
  • Elevated Plasma
  • Fluid Resuscitation