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Volume 3 Supplement 1

ESICM LIVES 2015

Open Access

Hemodynamics effects of adrecizumab in sepsis rat

  • A Blet1, 2, 3,
  • M Sadoune1,
  • E Polidano1,
  • R Merval1,
  • C Bernard1,
  • JL Samuel1 and
  • A Mebazaa1, 2, 3
Intensive Care Medicine Experimental20153(Suppl 1):A618

https://doi.org/10.1186/2197-425X-3-S1-A618

Published: 1 October 2015

Keywords

Septic ShockSevere SepsisMean Arterial PressureHemodynamic EffectVascular Dysfunction

Introduction

Sepsis and septic shock still represent major health issues, with persisting high morbidity and mortality rates in critically ill patients. Cardiac dysfunction[1] occurs frequently during severe sepsis.

Adrenomedullin (ADM) has been identified as a key mediator in vascular tone regulation[1]. A newly developed anti-ADM antibody Adrecizumab (ADZ) may improve hemodynamic dysfunction during resuscitated murine, cecal ligation and puncture (CLP)-induced septic shock[2].

Objectives

To determine the beneficial role of ADZ on hemodynamic impairment in a rat model of acute sepsis.

Methods

For induction of polymicrobial sepsis, cecal ligation and puncture (CLP)[3] was performed in Wistar male rats. ADZ (2 mg/kg) was injected IV 24 h after the surgery. There were 7 animals per group. Invasive blood pressure and cardiac function (by echocardiography) were assessed until 2 hours after ADZ injection. Statistical analysis was performed with 2 ways ANOVA.

Results

Septic rats had lower mean arterial pressure (MAP) (p < 0.0001) 24 h after surgery (at baseline) compared to sham. Septic animas with ADZ had a trend to have a greater MAP. A transient decrease of SF was observed 15 min and 1 h after injection of ADZ (p = 0.05). On the other hand cardiac output seems to be increased by ADZ (p = 0.61).

Conclusions

During sepsis in rats, treatment by ADZ seems to have a beneficial effect on cardiac and vascular dysfunction. These preliminary results need to be confirmed in preclinical and clinical studies.
Figure 1

[Mean arterial pressure]

Grant Acknowledgment

Adrenomed

Authors’ Affiliations

(1)
Inserm, Paris, France
(2)
Department of Anesthesiology and Critical Care and Burn Center, GH Lariboisière Saint-Louis, APHP, Paris, France
(3)
University Paris VII Denis Diderot, Paris, France

References

  1. Nishio K, et al: Critical Care Medicine. 1997, 25 (6): 953-7. 10.1097/00003246-199706000-00010.PubMedView ArticleGoogle Scholar
  2. Struck , et al: Intensive Care Med Expe. 2013, 1: 3-10.1186/2197-425X-1-3.View ArticleGoogle Scholar
  3. Rittirsch , et al: Nature Protocols. 2009, 4: 31-36. n°1PubMedPubMed CentralView ArticleGoogle Scholar

Copyright

© Blet et al.; 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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