- Poster presentation
- Open Access
Post-operative immune suppression is reversible with interferon gamma and independent of IL-6 pathways
© Longbottom et al.; 2015
- Published: 1 October 2015
- Interferon Gamma
- Grant Acknowledgment
- Overnight Hospital Stay
- Donor PBMCs
- Control Peripheral Blood
The post-operative period is characterised by increased IL-6 production and clinical features of immune suppression. In vitro anti-inflammatory actions of IL-6 are mediated through suppression of interferon gamma (IFNγ) . The clinical significance of IL-6 in mediating post-operative immune suppression remains unclear.
To evaluate the role of IL-6 pathways in post-operative immune suppression and the reversibility of this phenomenon.
Patients over 45 years old undergoing elective surgery involving the gastrointestinal tract and requiring at least an overnight hospital stay were recruited. The primary outcome was hospital-acquired infection. IL-6 and IFNγ levels were assayed using ELISA preoperatively and at 24 and 48 hours. Pooled healthy control peripheral blood mononuclear cells (PBMCs) were cultured in perioperative serum and CD14+HLA-DR (mHLA-DR) geometric mean florescent intensity (MFI) measured in the presence and absence of interferon gamma (IFNγ) and IL-6 neutralising antibody. Data were analysed with non-parametric statistics.
IL-6 levels increase following major surgery and are associated with an increased susceptibility to post-operative infections. Serum obtained from post-operative patients induces an immunosuppressive response through an IL-6 independent pathways which is reversible with IFNγ treatment.
Characteristics of patients developing infections and those remaining infection free following scheduled abdominal surgery.
N = 44 (37%)
N = 75 (63%)
66 (59 - 75)
64 (56 - 71)
Male sex (%)
Current smokers (%)
Cancer diagnosis (%)
Preoperative Immunosuppression (%)
Duration of operation (minutes)
243 (176 - 312)
195 (142 - 295)
Data are described as median with interquartile range with percentages in parenthesis