- Poster presentation
- Open Access
Iron deficiency, independently of anaemia, is associated with decreased cardiac systolic function and alteration of myocardial mitochondrial metabolism in a mouse model
© Gaillard et al.; 2015
- Published: 1 October 2015
- Left Ventricular Ejection Fraction
- Iron Deficiency
- Forced Swimming Test
- Mitochondrial Metabolism
- Racing Time
Iron deficiency (ID) is very frequent in cardiac patients and has been associated with altered cardiac function. The correction of ID in patients with heart failure improves clinical symptoms, in patients with or without anemia [1, 2]. Alteration of mitochondrial metabolism due to ID may be responsible for these observations.
We developed a mouse model of ID without anaemia to assess the link between ID and cardiac function, to characterize the mitochondrial metabolism and to assess the effect of iron treatment.
4 groups of C57BL/6 mices were compared : ID (obtained by a first retro-orbital puncture followed by 3 weeks of low iron diet); control mices (CT group). These mice received either an intraperitoneal saline injection or a single dose of 15 mg/kg carboxymaltose ferric at week 1 (ID+I and CT+I groups). Effort capacity was measured using a Rotarod (ie by assessing the racing time) and a forced swimming test (a load equal to 5% of the body weight was attached at the base of the tail and the duration of swim was measured). Left Ventricular Ejection Fractions (LVEF) were measured using transthoracic echocardiography done by a cardiologist blinded to the iron status. After sacrifice at W3, haemoglobin (Hb) was measured and activities of the different mitochondrial respiratory chain complexes were measured by spectrophotometry on myocardial muscle. Myoglobin and complex I were quantified using BN-PAGE.
In this mice model of ID without anaemia, we observe early decrease in exercise capacity and LVEF, which could be linked to a decrease in mitochondrial complexe I activity and amount. All these observations were reversed by a single iron injection.
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