Fig. 2
From: Heparin-binding protein is important for vascular leak in sepsis
HBP increases the permeability of human endothelial cell monolayers. a EA.hy926 cells were grown to confluence on permeable supports and stimulated with HBP. TEER across the filter was monitored over time. The overall difference was determined by two-way ANOVA (treatment effect P = 0.001, time effect P < 0.001). b HRP was added to the top chamber, and HRP passage was monitored over time. Values are normalized to the TEER of empty inserts. The overall difference was determined by two-way repeated measures ANOVA (treatment effect P = 0.009, time effect P < 0.001). In both experiments, Sidak’s multiple comparison post hoc test was used to compare HBP treatment and control at each time point. Error bars are standard error of the mean, n = 3. Some error bars are not visible due to scale. *P < 0.05, **P < 0.01, ***P < 0.001