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Intensive Care Medicine Experimental
Fig. 4 | Intensive Care Medicine Experimental

Fig. 4

From: Assessment of immune organ dysfunction in critical illness: utility of innate immune response markers

Fig. 4

Flow-cytometric assessment of monocytic HLA-DR expression. Upper row: after staining of EDTA samples with specific antibodies, HLA-DR expression is assessed on CD14+ monocytes by flow cytometry. Lower row: (left) Quantibrite™-PE beads are used to calculate a calibration curve (middle) for HLA-DR assessment on CD14+ monocytes. (Right) mean fluorescence intensity (MFI) values for HLA-DR on monocytes are converted in a given sample to molecules per cell using the calibration curve

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