0082. Early circulating lipid and cytokine profiles prognosticate in a rat model of faecal peritonitis
© Khaliq and Singer; licensee Springer. 2014
Published: 26 September 2014
In stress states, catecholamines induce lipolysis and insulin resistance with hyperglycaemia. Lipid profiles differ between surviving and non-surviving septic patients [1, 2] but, hitherto, little attention has been paid to this finding and its significance remains unknown. We used a previously characterized 72h fluid-resuscitated rat model of faecal peritonitis where prognostication can be made with high sensitivity and specificity as early as 6h from heart rate or stroke volume .
To determine the relationship between early changes in plasma cytokine and metabolic profiles, and their prognostic significance.
Under general anaesthesia male Wistar rats (325±15g) underwent tunneled insertion of carotid arterial and jugular venous lines, followed by i.p. injection of 4µl/g faecal slurry. They were then woken and attached to a swivel-tether system allowing free movement in their cage with, from 2h, fluid resuscitation (1:1 mix of 5% dextrose:Hartmann's) at 10ml/kg/h. An echocardiography-measured HR cut-off of 460 bpm was used to classify animals into predicted survivors or non-survivors. At 6h, animals were sacrificed for blood and tissue sampling. We here report plasma levels of IL-6, IL-10, and a metabolic profile using blood gas analysis, ELISA and enzymatic colorometric testing.
Predicted survival (n=6)
Predicted non-survival (n=6)
0.94 ± 0.23
3.70 ± 0.83*
0.33 ± 0.05
0.30 ± 0.12
6.8 ± 0.7
6.9 ± 0.6
1.9 ± 0.5
1.6 ± 0.5
HDL cholesterol (mmol/L)
0.88 ± 0.04
0.73 ± 0.07*
LDL/VLDL cholesterol (mmol/L)
0.50 ± 0.03
0.39 ± 0.03*
1.12 ± 0.04
0.75 ± 0.08*
In this long-term rat model of faecal peritonitis, predicted non-survivors had a significantly different IL-6 and lipid profile as early as 6 hours after sepsis. IL-6 impacts on lipid metabolism  but the relationship in sepsis has not, to our knowledge, been previously described. The impact of early hypolipidaemia on outcome warrants further investigation.
UK Intensive Care Foundation and NIHR
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